Gap Crossing

This device was developed for mice ans allows to study their motivation to jump from a platform to another one in order to obtain a food answer. Thanks to the high-frequency camera, whisker movments can be observed and analyzed.

GAP CROSSING

This device was developed for mice and allows to study their motivation to jump from a platform to another one in order to obtain a food reward. Thanks to the high-frequency camera, whisker movments can be observed and analyzed.

gap crossing

Photo credit : “Deficits in Tactile Learning in a Mouse Model of Fragile X Syndrome

 

Apparatus

The Gap Crossing is equiped by:

  • A high structure composed by 2 different platforms. The gap between those can be adjusted by users between 0 to 10 cm,
  • A “reward platform” where is located an automatic food dispensers (with a purge system),
  • An automatic door which control the access to the distal platform (i.e. reward platform),
  • An infrared beams frame to measure the animal progress into the system,
  • A sound generator with adjustable decibels,
  • A high-frequency camera (300 fps) that allows to film animal’s jump between both platforms,
  • A floor with infrared backlight,
  • An electronic TTL pulse generator, which allows synchronization with electrophysiology or optogenetics (open and closed-loop activation),
  • A PC-computer to run our softwares,
  • An electronic interface.

The apparatus is placed in a ventilated wooden cubicle, to isolate the system form the environment.

 

Gap crossing 0cm  Gap 0-10cm

Courtesy of Dr F. Gambino, cf. AMPAR-Dependent Synaptic Plasticity Initiates Cortical Remapping and Adaptive Behaviors during Sensory Experience

 

Software

Our POLY software allows:

  • Management of the experiment: opening the door, distribution of rewards,
  • Establishment a library of exercises and display in real-time of the behavioral events,
  • Secured recording of all the events, etc.

Our POLY_Files software calculates:

  • The locomotor activity,
  • Time spent in each area,
  • The number of times that the animal succeed,
  • The number of rewards obtained,
  • Delays of response…

All the results are displayed in a Microsoft Excel compatible datasheet.